《植物生理学报》 2009, 45(9): 875-877

微胚乳玉米籽粒发育期间非胚部位(胚乳)中的腺苷二磷酸- 葡萄糖焦磷酸化酶活性变化

甘凤琼, 吴子恺*

广西大学农学院, 南宁530005

通信作者：吴子恺；E-mail: wuzikai@gxu.edu.cn；Tel: 0771-3235612

摘　要：

检测微胚乳玉米非胚部位(胚乳)中腺苷二磷酸 - 葡萄糖焦磷酸化酶(ADPG-PPase)的活性结果表明: 微胚乳玉米的非 胚部位(胚乳)中 ADPG-PPase 活性在授粉后 21~28 d 达到峰值, 而对照的玉米品种 ‘ 高油 115’ 的 ADPG-PPase 活性在授粉 后 14~21 d 达到峰值, 滞后约 7 d。‘ 高油 115’ 非胚部位(胚乳)中 ADPG-PPase 活性最大值极显著高于微胚乳玉米, 其单粒 ADPG-PPase 活性最大值为微胚乳玉米的 2.2~3.6 倍, 其每克干重 ADPG-PPase 活性最大值则为微胚乳玉米的 2.4~3.8 倍。

关键词：微胚乳玉米; 腺苷二磷酸-葡萄糖焦磷酸化酶; 酶活性; 非胚部位(胚乳)

收稿：2009-05-31   修定：2009-08-24

资助：国家自然科学基金(30671307)和广西科学研究与技术开发计划(桂科攻0632001-1-I)。

Changes of ADP-Glucose Pyrophosphorylase Activity in the Non-Embryo (Endosperm) of Micro-Endosperm Maize (Zea mays L.) during Grain Development

GAN Feng-Qiong, WU Zi-Kai*

College of Agriculture, Guangxi University, Nanning 530005, China

Corresponding author: WU Zi-Kai; E-mail: wuzikai@gxu.edu.cn; Tel: 0771-3235612

Abstract:

The ADP-glucose pyrophosphorylase (ADPG-PPase) activity in non-embryo (endosperm) of microendosperm maize (Zea mays) was measured. The results showed that: the ADPG-PPase activity in non-embryo (endosperm) of micro-endosperm maize reached to the maximum during 21–28 d after pollination. It was late for 7 d than control Z. mays cv. ‘Gaoyou 115’, which ADPG-PPase activity reached to the maximum during 14–21 d after pollination. The maximum ADPG-PPase activity in non-embryo (endosperm) of cv. ‘Gaoyou 115’was significantly higher than that in micro-endosperm maize, such as, the ADPG-PPase activity of cv. ‘Gaoyou 115’ in single kernel was 2.2–3.6 times higher than micro-endosperm maize, and the ADPG-PPase activity per gram dry weight of cv. ‘Gaoyou 115’ was 2.4–3.8 times higher than micro-endosperm maize.

Key words: Zea mays ‘micro-endosperm maize’; ADPG-PPase; enzyme activity; non-embryo (endosperm)